Anti-Tenacibaculum maritimum monoclonal antibody, IgG2a

Description

The monoclonal antibody (Mab) against Tenacibaculum maritimum is species-specific. The specificity of the Mab has been tested against a range of fish bacterial pathogens including different Tenacibaculum sp. The Mab is of an IgG2a isotype.

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  Lane 1: Molecular weight markers. Lane 2: SDS-PAGE of the protein A-purified Mab. Coomassie staining.Lane 3: Western blot (WB). MAb detection using an anti-mouse IgG specific polyclonal antibody (whole molecule). Lane 4: WB. Tenacibaculum maritimum reduced lysate transferred to a PVDF membrane was detected using CETGA-101 Mab (2 μg/mL) and anti-mouse IgG specific antibody as secondary antibody.

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  Immunofluorescence. Fluorescence microscopy images (Zeiss axiolab 5) of T. maritimum from a 48 h FMM broth culture, incubated with the mAb CETGA101, detected using an anti-mouse IgG-FITC secondary antibody. The nuclei were stained with DAPI.